How does an analysis work?

 How does an analysis work?

A microplastics analysis at NanoPlasticsLab follows a strictly controlled, ISO 9001–compliant workflow. Every step is documented and designed to ensure contamination-free, reproducible, and auditable results.

    • On request, we provide a complete sampling kit: pre-rinsed and blank-tested glass bottles suitable for different matrices.

    • Both the bottles and the blanks can be verified individually if required.

    • Transport is organized so that no additional contamination risk is introduced.

    • All steps use only MilLi-Q water, filtered multiple times through 200 nm membranes and continuously monitored.

    • Standard filtration is performed on specially coated membranes that:

      • show no Raman background, and

      • are optimized for the detection of nanoparticles.

    • Depending on the matrix, we apply cascaded filtration: multiple filters with decreasing pore sizes to pre-filter and fractionate particles.

    • Staff wear cotton clothing, gloves, and protective gear to prevent plastic shedding.

    • All work is performed in laminar-flow clean air conditions.

    • Water, filters, and glassware are routinely checked.

    • Blanks are taken before and after each measurement to monitor background levels.

    • All staff receive regular training to maintain the highest standards.

    • After preparation, particles remain directly on the coated membranes for measurement — avoiding transfer steps that might cause losses.

    • First, the sample is scanned with DeepMorph Imaging, an AI system that detects shape, size, and particle count.

    • Then, each identified particle is analyzed by Raman spectroscopy (RamanMetrix) and compared against our polymer library for accurate identification.

    • Standard data analysis: Principal Component Analysis (PCA) and Pearson correlation – transparent, auditable, and robust.

    • Optional (R&D only): advanced machine learning models (CNNs, Random Forests, etc.) for pattern recognition and exploratory insights.

    • Suspicious material (e.g., from packaging or production sites) can be submitted.

    • Using a specialized machine-learning database, we can assign likely sources of microplastic particles.

    • Every measurement includes negative and positive controls.

    • Methods are regularly checked with spiked reference samples (irregular PET, PE, PS, PVC nanoplastics).

    • On request, we validate methods for the customer’s matrix to ensure auditable and forensically robust data.

    • Customers receive a detailed PDF report including:

      • Total number of particles (absolute & normalized)

      • Size distribution & morphology (form, length, diameter)

      • Polymer identification (PET, PE, PS, PVC, etc.)

      • Optional: statistical analyses (PCA, Pearson correlation)

    • Optional: Compliance report ready for integration into quality systems (e.g., cGMP).

    • Raw data can also be provided for research or publication purposes.